This experiment looked at how substrate concentration can affect enzyme activity. Hydrogen peroxide solution of '5 volume' concentration is low hazard, but it will probably need to be prepared by dilution of a more concentrated solution which may be hazardous. An exact cutter that would make equal potato slice sizes. Materials: Potato Test tube or other small container Hydrogen Peroxide 1. Oxygen is soluble in water, but dissolves only slowly in water at normal room temperatures. .
Haliotis discus discus, a type of shellfish, for example, has catalase which works at an optimum pH of 10. Fill pot half way with water and keep it on stove until temperature is 50°C using thermometer. Testing for enzymes Class practical Hydrogen peroxide is used to detect the presence of enzymes in liver, potato and celery, which catalyse the decomposition of hydrogen peroxide, by detecting the presence of the oxygen gas formed. The concentration of substrate for example will affect the rate of reaction in a positive way until all the active sites are occupied. Preparation a Make just enough diluted hydrogen peroxide just before the lesson.
Why that is, no one seems to know. H2O2 is a really reactive species and can damage cells and issues by oxidising them. Potato and Hydrogen Peroxide Enzyme Lab Experimental Variables: Question: How do environmental factors affect enzyme function? Conclusion Through our experimentation the reaction between the enzyme catalase and hydrogen peroxide was tested. The average mL of O2 for boiled potatoes was 1. However, while ammonia had no reaction with the catalase in the potato in the solution, after the potato was removed, there was a texture change. Hydrogen peroxide H2O2 is a by-product of respiration and is made in all livin g cells. Ask students to explain how values over 22 cm 3 could happen.
I was wondering if you could explain the theory of this. All enzymes have an active site. Put the syringe in place in the bung of the flask, but do not push the plunger straight away. Less foam means it is not working as well, and no foam means it is not working at all. Reassemble the apparatus, refill the measuring cylinder, and repeat from g to j with another concentration of hydrogen peroxide.
The mashed potato had more enzymes open to combine with the substrate; therefore there was a greater release of products. When the potato is cut into eights I predict that there would be a faster reaction occurring as now the potato is doubled there would be plenty of surface area within the potato and the collision would be doubled due to the amount being cut to eights. In Part C, we altered the pH of the solution by adding drops of diluted Hydrochloric Acid to see if it had an affect on the rate of reaction in catalyses on pieces of liver. Hydrogen peroxide is a molecule that can attack sulphur atoms and metal ions in protein molecules, and with the aid of iron ions is potentionally mutagenic. So therefore I predict that the reaction would be faster then the cut in half potato. It is an incredibly efficient enzyme where one catalase molecule can convert millions of hydrogen peroxide molecules each second. Procedure: 1 Fill 4 100mL- graduated cylinders with 60 mL of hydrogen peroxide.
Conclusion: In this conducted experiment, the variable measured was the duration of the reaction on a raw sweet potato. This is true because in the reaction, the hydrogen peroxide is observed to have gone through a chemical change; turning into water molecules and dioxide molecules, whereas the liver stayed the same. The concentration of the inhibitor will have no effect on the rate of this reaction. Push the plunger on the syringe and immediately start the stopclock. Although our hypothesis was primarily correct in that hydrogen peroxide would have the longest reaction time, we did not expect for ammonia and vinegar to have absolutely no reaction with the catalase at all. Therefore beyond a certain temperature, the rate of reaction will decrease. During our experiment we encountered some things that could have created some slightly off data.
Large particles have a small surface area due to their volume, so fewer particles are exposed and are available for collisions. Repeat five times, and record your data in your data table, including an average for this test. Background Information Enzymes such as Catalase are protein molecules which are found in living cells. Each enzyme just performs one particular reaction so they are all very specific. Begin with about 40 grams of peeled potato and blend it in about 100ml of water and ice.
This enzyme finds applications in Research and Clinical Chemistry. Catalase works to speed up the decomposition of hydrogen peroxide into oxygen and water. More or less than 10 mL of hydrogen peroxide could alter results because maybe there would be more or less hydrogen peroxide for the catalase to react with. Rate of Reaction Between Hydrogen Peroxide and the Potato Prediction The predictions for the rate of reaction, between the Hydrogen Peroxide and the potato. All these reactions were properly shown in our own conducted experiment. Catalase breaks down hydrogen peroxide, which is toxic, into 2 safe substances- water and oxygen, by speeding up a reaction.
When there's a small amount of collision occurring this would increase the time taken and so therefore the reaction would be slow. For the potato which would be cut into two I predict that the reaction would be slow once again due to the large particle sizes. Once the same amount of the substrate was added to the potato there was a slight fizzy and bubbly reaction thus earning the rating of 2. Explain why there is a difference in the rates of reaction between the liver and the potato Liver contains more of the enzyme catalase, which breaks down hydrogen peroxide. Vinegar however, had the absolute same reaction in and out of the solution as the control group.
Larger particles decreased the rate of reaction because they require more energy to break down. Since our potato was raw, the catalase in the enzyme of the potato was not altered or damaged at all. It also finds diverse industrial applications in textiles, waste treatment, cosmetics and as a disinfectant agent. Quantitative Data: Potato and hydrogen peroxide bubble height Purpose: To see peroxide effects on the enzymes in the potatoes. Hypothesis The hypothesis is that catalase activity will increase exponentially with higher concentrations of hydrogen peroxide until all catalase active sites are filled, in which case the… 1577 Words 7 Pages The effect of varying the substrate concentration of hydrogen peroxide on the rate of enzyme activity of catalase from potato peel. The apple sample is simply carbohydrates meant as food for the apple seed and will have no enzymes in it, thus no reaction was observed. For this experiment I will predict what the affect of the concentration, particle size and temperature would be.